NATlab Technology
INNOVATIVE TECHNOLOGY USING ROLLING CIRCLE AMPLIFICATION (RCA)
The diagnostic assay inside the cartridge is based on a patented rolling circle amplification (RCA) protocol, rather than PCR, enabling multiplex detection of up to 100 pathogens by a proprietary carbon array sensor embedded in the cartridge.
Isothermal amplification using RCA instead of PCR provides superior accuracy, sensitivity and speed.
NATlab Key Advantages
Superior accuracy
High sensitivity and specificity enabled by proprietary rolling circle amplification assay inside the cartridge
High multiplexing
Allowing simultaneous detection of 100 different pathogens/genomic targets in a single test
Rapid results
30-45 minutes analysis, enabling its use at near-patient setting
Fully automated
Allowing operation by minimally trained non-professional personnel
Easy panel modification
Allowing fast response to new emerging pathogens and strains
Connectivity
NATlab device connectivity and geographical mapping of test results for outbreak detection and management
The NATlab device
The NATlab system includes a compact benchtop device and a dedicated syndromic cartridge.
This system enables performing the RCA-based pathogen detection assay in a ‘Sample-to-Answer’ mode.
Patented amplification reaction using RCA
The proprietary RCA reaction can detect as low as single-digit copy number of a pathogenic target.
The technology is extensively patented as a combined technology of the NATlab cartridge, molecular assay and instrument.
Sample
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Genomic DNA extraction
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Pathogen selective gDNA isothermal pre-amplification
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Circular probe hybridization with target amplicons
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Ligation of positive circular probes
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Elimination of non-ligated probes & gDNA
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RCA Amplification of positive probes
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Cutting RCA products to short oligos
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Hybridization and detection of amplified targets with carbon array probes
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Assay report
Syndromic microfluidic Cartridges
The patented cartridge is a closed disposable system that houses all the chemical and biological reagents required for isolation, RCA amplification, and multiplex detection of pathogenic DNA/RNA
The proprietary carbon array sensor inside the cartridge enables fast and efficient multiplex detection of DNA/RNA targets from multiple bacteria, viruses or fungi.
Sample to Answer operation mode allowed by the patented microfluidic cartridge, enables the operation of the system by non-professional minimally trained personnel
Dedicated syndromic cartridges (panels) for each condition disease assures comprehensive and fast diagnosis of the pathogen
Easy panel modification is enabled by the proprietary sensor technology and the patented isothermal amplification assay. This allows fast response to new emerging pathogens and strains
NATlab Cartridge Key Advantages
Sample-to-Answer:
insert unprocessed sample into the cartridge and then into the analyser
Carbon array multiplex sensor technology enabling comprehensive testing capabilities
Based on patented isothermal amplification protocol - cost effective, rapid and accurate
Syndromic approach - dedicated cartridge per syndrome
Stored and operates at room temperature
Optional feature for monitoring outbreaks
The NATlab system harbors an optional feature for connectivity between NATlab units located in various sites and geographical mapping of test results, thereby enabling outbreak detection and management of pandemic.
IVD Innovative technologies
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Dedicated syndromic cartriges solution